Complementary dual-modal immunosensing strategy for accurate detecting zearalenone based on bimetallic NiFe-Prussian...

In this work, a complementary dual-modal immunosensing strategy was fabricated for the sensitive and selective detection of zearalenone (ZEN) by integrating immunochromatographic and electrochemical biosensing methods. For this, bimetallic NiFe Prussian blue analogue (NiFe-PBA) was simultaneously employed as a matrix for labelling ZEN-targeted antibody and the sensitive layer for anchoring antibody to construct an efficient immunochromatographic immunosensor (ICI) and electrochemical immunosensor (ECI), respectively. Due to porous network, good functionality, high bioaffinity, and rich metal redox, NiFe-PBA-based complementary dual-modal immunosensor exhibited low limit of detection, high selectivity and stability, and accepted practicality. Particularly, the developed ECI illustrated fast response, portability, and potentially commercial application. Coupling the ICI and ECI sensing strategy can markedly enhance the accuracy and sensitive detection of ZEN in complicated environments. This dual-modal ICI-ECI immunosensing strategy exhibits significant potential for the precise detection of mycotoxin in various agricultural products and improve the quality control for foods.