ELISA for Detection of Total IgG Against YF 17DD

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Ana Carolina R A Cajaraville1

  • 1Laboratory of Virological Technology (LATEV), Institute of Technology in Immunobiologicals (Bio-Manguinhos), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil. anacarolina.reis@bio.fiocruz.br.

Methods in Molecular Biology (Clifton, N.J.)
|April 18, 2025
PubMed

The ELISA technique has attracted widespread interest as a sensitive, efficient, safe, and inexpensive way to measure antigen-antibody reactions since its description in 1971 to current days. Here it is described an indirect ELISA protocol to detect anti-YF 17DD antibodies as an important tool to evaluate immunogenicity to yellow fever vaccine candidates.

Keywords:
Anti-YF 17DDEndpoint ELISAIndirect ELISATotal IgG antibodies

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