Mapping RNA-Protein Interactions via Proximity Labeling-Based Approaches
Yongzuo Chen1,2, Yuxin Chen1,2, Wei Qin1,2,3,4,5
1School of Pharmaceutical Sciences, Tsinghua University, Beijing, 100084, China.
Chemistry, an Asian Journal
|April 18, 2025
Summary
Proximity labeling offers a crosslinking-free method to study RNA-protein interactions. This review explores new RNA-centric and protein-centric techniques for profiling these vital cellular interactions.
Area of Science:
- Molecular Biology
- Cellular Biology
- Biochemistry
Background:
- RNA-protein interactions are crucial for cellular functions.
- Conventional crosslinking methods can create artifacts.
- Studying these interactions in vivo is challenging.
Purpose of the Study:
- To review recent advancements in proximity labeling for RNA-protein interaction studies.
- To highlight both RNA-centric and protein-centric strategies.
- To provide insights into understanding RNA-protein dynamics.
Main Methods:
- Proximity labeling techniques (crosslinking-free).
- RNA-centric strategies.
- Protein-centric strategies.
Main Results:
- Proximity labeling provides artifact-free insights into RNA-protein interactions.
- Recent methods offer enhanced profiling capabilities.
- These techniques advance the study of RNA-protein dynamics.
Conclusions:
- Proximity labeling is a powerful alternative to traditional methods.
- Innovative strategies are improving the understanding of RNA-protein interactions.
- Further application of these methods will enhance biological insights.
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