Algal-bacterial granules for circular bioeconomy: Formation mechanisms and biotechnological applications

Cyanobacteria and microalgae are sustainable and renewable biocatalysts for solar energy harvesting, recovering nutrients from wastewater, reducing greenhouse gas emissions from wastewater treatment plants (WWTPs) and enable creation of a sustainable circular bioeconomy. Pure and axenic cultures of photosynthetic microorganisms have been widely studied for synthesizing bio-based products through improving the metabolic pathways via genetic engineering. However, pure cultures suffer from contamination and separation challenges when considered for environmental applications. Mixed microbial communities comprising of photosynthetic organisms and bacteria in the form of either flocs or granules have recently received a lot of attention due to their potential contribution to wastewater treatment, environmental sustainability and circular bioeconomy. The advantages of algal-bacterial granules (ABG) in WWTPs include effective elimination of contaminants and nutrients, reduction in aeration requirement, and production of biomass feedstock for downstream processing. Although ABG are an attractive option for energy positive wastewater treatment, it is not yet matured as technological option for deployment in full-scale WWTPs. Moreover, several aspects of ABG including synergistic metabolism, granulation mechanisms, granular stability, bioreactor operating conditions, cell-cell interactions, extracellular polymeric substances and bio-based products deserve more intense research. This article provides a detailed overview of algal-bacterial communities, their occurrence in natural environments, ABG cultivation in engineered settings, potential biotechnological applications and the recent progress made towards sustainable biological wastewater treatment and circular bioeconomy.