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Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

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Updated: May 11, 2025

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Published on: February 15, 2019

AFED, a comprehensive resource for Aspergillus flavus gene expression profiling.

Brian M Mack¹, Matthew D Lebar¹

¹Food and Feed Safety Research, Southern Regional Research Center, Agriculture Research Service, United States Department of Agriculture (USDA), 1100 Allen Toussaint Blvd, New Orleans, LA 70124, United States.

Database : the Journal of Biological Databases and Curation

|April 18, 2025

Summary

This summary is machine-generated.

The Aspergillus flavus expression database (AFED) provides a centralized resource for A. flavus gene expression data. This tool aids researchers in understanding aflatoxin biosynthesis and developing control strategies for this food security threat.

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Last Updated: May 11, 2025

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Published on: February 15, 2019

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Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Published on: January 13, 2016

Area of Science:

Mycology
Genomics
Bioinformatics

Background:

Aspergillus flavus is a significant fungal pathogen impacting food security through aflatoxin contamination.
Complex regulation of aflatoxin biosynthesis necessitates centralized gene expression data.
Existing resources lack comprehensive expression data for A. flavus across diverse conditions.

Purpose of the Study:

To develop a centralized database for Aspergillus flavus gene expression data.
To facilitate the study of A. flavus biology and aflatoxin biosynthesis regulation.
To support the development of strategies for controlling A. flavus.

Main Methods:

Collected and processed public RNA sequencing data from 604 A. flavus samples across 52 experiments.
Quantified gene expression abundance using RNA sequencing data.
Developed a web-based interface for accessing and visualizing expression profiles and co-expression networks.

Main Results:

Created the Aspergillus flavus Expression Database (AFED) with integrated gene expression data.
AFED allows visualization of gene expression profiles via interactive bar plots and heatmaps.
A gene co-expression network is available for identifying co-expressed genes and their functional enrichment.

Conclusions:

AFED serves as a valuable resource for researchers studying Aspergillus flavus.
The database enables comprehensive analysis of gene expression across various conditions and life stages.
AFED supports deeper understanding of aflatoxin biosynthesis and aids in developing mitigation strategies.