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Updated: May 14, 2025

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A tomato mottle mosaic virus-based vector system for gene function studies in tomato.

Zhi-Yong Yan1, Carlos Kwesi Tettey1, Hua-Yu Ma1

  • 1College of Plant Protection, Shandong Agricultural University, Tai'an, 271018, PR China.

Virology
|April 19, 2025
PubMed
Summary

Researchers developed a new plant virus vector for tomatoes. This efficient Tomato mottle mosaic virus (ToMMV) vector enables systemic gene expression and protein studies in plants like tomatoes.

Keywords:
TobamovirusTomatoTomato mottle mosaic virusVirus vector

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Area of Science:

  • Plant virology
  • Molecular biology
  • Crop science

Background:

  • Plant virus vectors are valuable for protein expression and gene function studies.
  • Systemic viral vectors for tomatoes are underdeveloped, limiting research.
  • Tomato mottle mosaic virus (ToMMV) is a potential candidate for vector development.

Purpose of the Study:

  • To develop a novel ToMMV-based viral vector for systemic gene expression in plants, particularly tomatoes.
  • To demonstrate the vector's utility for expressing reporter genes and functional genes.
  • To establish an efficient tool for gene function analysis in Solanum lycopersicum.

Main Methods:

  • Modification of a ToMMV infectious clone by replacing the coat protein (CP) gene with Green Fluorescent Protein (GFP).
  • Introduction of a sub-genomic promoter and CP gene from another tomamovirus to enable systemic spread.
  • Insertion and expression of genes encoding Rosea1 transcription factor, bialaphos resistance protein, and β-glucuronidase (GUS).

Main Results:

  • The modified ToMMV vector successfully expressed GFP in systemically infected leaves of Nicotiana benthamiana and Solanum lycopersicum.
  • Expression of Rosea1 induced anthocyanin biosynthesis in systemic leaves of both plant species.
  • The vector conferred herbicide resistance by expressing the bialaphos resistance gene.
  • A 68 kDa β-glucuronidase was successfully expressed in systemic leaves and roots.

Conclusions:

  • A ToMMV-based viral vector was successfully engineered for systemic gene expression in plants.
  • This vector serves as a simple and efficient tool for gene function studies in tomatoes and other Solanum species.
  • The developed vector broadens the application of plant virus vectors in crop research.