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Updated: May 10, 2025
Multimodal Study of Murine Cardiovascular Remodeling: Four-Dimensional Ultrasound and Mass Spectrometry Imaging
Published on: January 10, 2025
Mass spectrometry-based characterisation of the cardiac microtissue metabolome and lipidome
Tara J Bowen1,2, Andrew R Hall3, Andrew D Southam1,4
1School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
Introduction:
The use of large, non-sample specific metabolite reference libraries often results in high proportions of false positive annotations in untargeted metabolomics.
Objective:
This study aimed to measure and curate a library of polar metabolites and lipids present in cardiac microtissues.
Results:
Untargeted ultra-high performance liquid chromatography-coupled mass spectrometry measurements of cardiac microtissue intracellular extracts were annotated by comparison against four spectral databases and a retention time library. The annotations were combined to create a library of 313 polar metabolites and 1004 lipids.
Conclusions:
The curated library will facilitate higher confidence metabolite annotation in mass spectrometry-based untargeted metabolomics of cardiac microtissues.
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