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Exploring UV Spectroscopic Approaches for Alendronate Sodium Quantification in Pharmaceutical and Biological Samples
Aashli Mary1, S Giridhar Reddy1, B Siva Kumar1
1Department of Physical Sciences, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Bengaluru, India.
Detecting alendronate sodium in biological and pharmaceutical samples is challenging. This review highlights UV-spectroscopic techniques, including derivatization and metal complexation, as economical and efficient methods for alendronate detection.
Area of Science:
- Analytical Chemistry
- Pharmaceutical Analysis
Background:
- Alendronate sodium is a bisphosphonate used for bone disorders like osteoporosis.
- Traditional detection methods are costly and complex.
- Alendronate lacks strong chromophores, complicating direct UV detection.
Purpose of the Study:
- To review UV-spectroscopic techniques for alendronate detection.
- To highlight economical and simplified analytical methods.
- To discuss strategies for enhancing alendronate's UV detectability.
Main Methods:
- Derivatization with chromogenic reagents (e.g., OPA, SNQS).
- Metal ion complexation (e.g., Fe3+, Cu2+, Ce4+) to enhance UV absorption.
- Fluorescence quenching/restoration using carbon dots and metal ions.
Main Results:
- Derivatization and metal complexation significantly improve UV detection sensitivity.
- Carbon dot-based fluorescence methods offer sensitive alendronate detection.
- UV-spectroscopic methods provide cost-effective and user-friendly alternatives.
Conclusions:
- UV-spectroscopic techniques are suitable for alendronate analysis in various matrices.
- These methods are crucial for therapeutic drug monitoring and quality control.
- Further development in derivatization and nanomaterial-based detection is promising.
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