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Neuropathy target esterase in human lymphocytes.

D Bertoncin, A Russolo, S Caroldi

    Archives of Environmental Health
    |May 1, 1985
    PubMed
    Summary
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    Measuring neuropathy target esterase (NTE) activity in lymphocytes can biomonitor organophosphate exposure. This blood test is reproducible and feasible for clinical use, but requires baseline values due to interindividual variation.

    Area of Science:

    • Biochemistry
    • Toxicology
    • Clinical Chemistry

    Background:

    • Neuropathy target esterase (NTE) activity in blood lymphocytes is a potential biomarker for organophosphate-induced delayed polyneuropathy.
    • Characterizing human lymphocyte NTE activity is crucial for validating its use as a biomonitor.

    Purpose of the Study:

    • To characterize human lymphocyte NTE activity in vitro.
    • To assess the feasibility and reproducibility of measuring lymphocyte NTE activity for biomonitoring organophosphate exposure.
    • To establish baseline values for lymphocyte NTE activity in a healthy population.

    Main Methods:

    • In vitro characterization of human lymphocyte NTE sensitivity to organophosphate inhibitors.
    • Standardized protocols for blood collection, storage, and processing.

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  • Development and validation of the NTE assay with assessment of method variability.

    • Main Results:

    • Human lymphocyte NTE sensitivity to organophosphate inhibitors mirrors that of the nervous system enzyme.
    • The NTE assay demonstrated good reproducibility with an average coefficient of variation of 8%.
    • Mean lymphocyte NTE activity in healthy Caucasians (n=108) was 11.5 ± 2.5 nMoles/min x mg protein, with no significant age or sex differences.

    Conclusions:

    • Measurement of lymphocyte NTE activity is a feasible and reproducible biomonitoring tool for organophosphate exposure.
    • Significant interindividual variation in NTE activity necessitates establishing appropriate baseline values for occupational exposure assessments.
    • The findings support the use of lymphocyte NTE activity assays in clinical settings to evaluate risks associated with delayed polyneuropathy.