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Plant protein-based active films via heteroprotein phase separation
Zhongyu Yang1, Jingyang Guo1, Gaoshang Wang1
1Laboratory of Food Proteins and Colloids, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Food Research International (Ottawa, Ont.)
|April 23, 2025
Summary
Biodegradable active films made from zein and soybean protein isolate (SPI) show improved mechanical and barrier properties. These plant-based films offer antibacterial and antioxidant benefits, extending the shelf life of fresh-cut apples.
Area of Science:
- Materials Science
- Food Science
- Biotechnology
Background:
- Growing plastic pollution necessitates eco-friendly alternatives like biodegradable active films.
- Plant proteins offer tunable properties for developing advanced packaging materials.
- Heteroprotein phase separation is a promising strategy for tailoring film characteristics.
Purpose of the Study:
- To develop a novel zein/soybean protein isolate (SPI) active film using heteroprotein phase separation.
- To investigate the co-encapsulation of Epigallocatechin-3-gallate (EGCG) and α-tocopherol within the film matrix.
- To evaluate the film's mechanical, barrier, antibacterial, and antioxidant properties for food packaging applications.
Main Methods:
- Preparation of zein/SPI composite films via heteroprotein phase separation.
- Transformation of SPI gels into microgels for the film matrix.
- Incorporation of zein nanoparticles and zein coacervate as fillers.
- Co-encapsulation of hydrophilic EGCG and hydrophobic α-tocopherol.
- Assessment of film morphology, mechanical strength, water vapor and oxygen permeability, and antioxidant/antibacterial activity.
Main Results:
- Composite films exhibited distinct phase separation modes and microstructures.
- Zein fillers significantly enhanced mechanical properties and reduced water vapor/oxygen permeability.
- The films demonstrated effective antibacterial and antioxidant activities.
- Application of the films slowed the oxidation of fresh-cut apples, extending shelf life.
Conclusions:
- A novel plant protein-based active film was successfully developed using heteroprotein phase separation.
- The composite films possess superior mechanical and barrier properties, along with functional bioactivity.
- This approach offers a sustainable and effective solution for active food packaging, reducing food waste and reliance on plastics.
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