Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bacterial Signaling

Bacterial Signaling

Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Integrating Epigenetic Memory and Plant Growth-Promoting Rhizobacteria -Mediated Signaling for Climate-Resilient Agriculture.

Journal of applied microbiology·2026

Same author

Programmable nanocarriers for precision plant engineering: converging nanotechnology, CRISPR, and next-generation breeding.

Frontiers in plant science·2026

Same author

Biosynthesis of antibacterial zinc oxide nanoparticles from endophytic Streptomyces werraensis.

Scientific reports·2026

Same author

Impact of Insecticide-Treated Nets on <i>Plasmodium falciparum</i> Infection Rates: A Meta-Analysis.

Tropical medicine and infectious disease·2026

Same author

An Insight into the Structural Modification and Mechanism of Ursolic Acid at C-3 and C-28: Strategy for Searching Anti-Cancer Therapeutics.

Current topics in medicinal chemistry·2026

Same author

Unraveling the synergistic effects of Bacillus cereus and different concentrations of silicon nanoparticles on wheat under rain-fed conditions of Pakistan.

Environmental geochemistry and health·2026

Search research articles

Related Experiment Video

Updated: May 10, 2025

A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling

A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling

Published on: July 22, 2017

Targeting Agrobacterium tumefaciens: A Computational Study on Quorum Sensing Inhibition.

Jayanthi Barasarathi¹, Kahkashan Perveen², Faheema Khan²

¹Faculty of Health and Life Sciences (FHLS), INTI International University, Nilai, Negeri Sembilan, Malaysia.

Journal of Basic Microbiology

|April 23, 2025

Summary

This summary is machine-generated.

Researchers identified N-phenylselenourea as a potent inhibitor of Agrobacterium tumefaciens quorum sensing (QS). This discovery offers a novel strategy to combat crown gall disease and improve plant health by disrupting bacterial communication.

Keywords:

N‐phenylselenourea TraR protein molecular docking plant microbiome transcriptomics

More Related Videos

Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana

Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana

Published on: April 19, 2014

Author Spotlight: Advancing Therapeutics to Treat Vibriosis in Humans and Aquatic Organisms

Author Spotlight: Advancing Therapeutics to Treat Vibriosis in Humans and Aquatic Organisms

Published on: May 31, 2024

Related Experiment Videos

Last Updated: May 10, 2025

A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling

A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling

Published on: July 22, 2017

Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana

Optimization and Utilization of Agrobacterium-mediated Transient Protein Production in Nicotiana

Published on: April 19, 2014

Author Spotlight: Advancing Therapeutics to Treat Vibriosis in Humans and Aquatic Organisms

Author Spotlight: Advancing Therapeutics to Treat Vibriosis in Humans and Aquatic Organisms

Published on: May 31, 2024

Area of Science:

Microbiology and Plant Pathology
Computational Chemistry
Agricultural Science

Background:

Crown gall disease, caused by Agrobacterium tumefaciens, leads to substantial agricultural losses via tumor formation.
Bacterial quorum sensing (QS) regulates A. tumefaciens virulence and pathogenicity.
QS influences plant-microbe interactions, affecting symbiosis and plant health.

Purpose of the Study:

To identify novel quorum sensing inhibitors (QSIs) targeting the TraR protein of A. tumefaciens.
To evaluate QSIs as a strategy for mitigating crown gall disease.
To explore the broader impact of QS disruption on plant-microbe interactions.

Main Methods:

Screening of potential QSIs using molecular docking and molecular dynamics (MD) simulations.
Analysis of protein-protein interactions and binding affinity to the TraR protein.
Gene Ontology (GO) enrichment analysis to assess pathway disruption.

Main Results:

N-phenylselenourea identified as a potent QSI with a binding affinity of -8 kcal/mol to TraR.
MD simulations confirmed stable binding of N-phenylselenourea within the TraR binding pocket, interacting with key residues Tyr53 and Asp70.
GO analysis indicated disruption of critical pathogenic pathways.

Conclusions:

QSIs, exemplified by N-phenylselenourea, offer a targeted approach to control A. tumefaciens infections.
Disrupting bacterial QS can mitigate crown gall disease and potentially enhance beneficial plant-microbe interactions.
This research supports the development of sustainable agricultural practices through QS modulation.