A PPR Protein RFCD1 Affects Chloroplast Gene Expression and Chloroplast Development in Arabidopsis
Tianming Tan1,2,3,4, Shengnan Xu1,2,3, Jiyun Liu1,2,3
1National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.
Abstract:
Chloroplast development is a highly complex process, involving many regulatory mechanisms that remain poorly understood. This study reports a novel PPR protein, RFCD1 (Regulation Factor of Chloroplast Development 1). Fluorescence localization analysis reveals that the N-terminal 60 amino acids of RFCD1 fused with GFP protein specifically direct the protein to the chloroplast. The knockout mutant of RFCD1 is embryo-lethal. RFCD1 RNA interference (RNAi) transgenic lines display chlorosis phenotypes and abnormal chloroplast development. Quantitative real-time PCR (qRT-PCR) showed that the expression levels of the plastid-encoded RNA polymerase (PEP) genes were significantly decreased in the RNAi lines. Furthermore, RNA blotting results and RNA-seq data showed that the processing of plastid rRNA was also affected in the RNAi lines. Taken together, these results indicate that RFCD1 might be involved in chloroplast gene expression and rRNA processing, which is essential for chloroplast development in Arabidopsis.
More Related Videos
10:10Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
Published on: May 29, 2010
10:20Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses
Published on: August 9, 2019
Related Concept Videos
Protein Transport to the Inner Chloroplast Membrane
Cell Signaling in Plants
Channel Rhodopsins
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Protein Transport to the Stroma
Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
Protein Transport to the Outer Chloroplast Membrane
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
Protein Transport to the Thylakoids
