Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell fate acquisition at a de novo developmental boundary in the maize leaf.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Single-Cell and Spatial Transcriptomic Analysis of Maize Embryo Development.

Cold Spring Harbor protocols·2025
Same author

Multiplexed transcriptomic analyzes of the plant embryonic hourglass.

Nature communications·2025
Same author

Integrative multi-omic analysis identifies genes associated with cuticular wax biogenesis in adult maize leaves.

G3 (Bethesda, Md.)·2024
Same author

Genetic analyses of embryo homology and ontogeny in the model grass Zea mays subsp. mays.

The New phytologist·2024
Same author

NAKED ENDOSPERM1, NAKED ENDOSPERM2, and OPAQUE2 interact to regulate gene networks in maize endosperm development.

The Plant cell·2023
Same journal

High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic <i>E. coli</i>.

Cold Spring Harbor protocols·2025
Same journal

Grain Quality in Maize.

Cold Spring Harbor protocols·2025
Same journal

High-Throughput Assay for Measuring Phytate and Available Phosphorus in Ground Maize Seed Samples.

Cold Spring Harbor protocols·2025
Same journal

Functional Genomic Analysis of Transposon Insertion Mutant Maize Plants from the UniformMu National Public Resource.

Cold Spring Harbor protocols·2025
Same journal

The UniformMu National Public Resource: Transposon<i>-</i>Induced Mutant Seeds for Functional Genomics Studies in Maize.

Cold Spring Harbor protocols·2025
Same journal

Insights from the Study of B<i>-</i>Cell Epitopes of a Microbial Pathogen by Phage Display.

Cold Spring Harbor protocols·2025
See all related articles

Related Experiment Video

Updated: May 10, 2025

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
10:08

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

9.4K

Single-Cell and Spatial Transcriptomic Analysis of Maize Embryo Development: A Sample Preparation Protocol.

Hao Wu1, Michael J Scanlon2

  • 1School of Integrative Plant Science, Plant Biology Section, Cornell University, Ithaca, New York 14853, USA haowu@njau.edu.cn.

Cold Spring Harbor Protocols
|April 23, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a new protocol for analyzing maize embryo development using single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics. These advanced methods enable high-resolution investigation of gene expression during early corn plant development.

More Related Videos

Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging
06:11

Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging

Published on: September 22, 2023

2.7K
Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
08:33

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Published on: August 5, 2020

7.9K

Related Experiment Videos

Last Updated: May 10, 2025

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
10:08

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

9.4K
Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging
06:11

Author Spotlight: Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of Maize Leaf Primordia for Fluorescence and Confocal Imaging

Published on: September 22, 2023

2.7K
Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
08:33

Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Published on: August 5, 2020

7.9K

Area of Science:

  • Plant Biology
  • Developmental Biology
  • Genomics

Background:

  • Maize is a vital crop for food, feed, and industry, necessitating research into its development for improved yields.
  • Understanding maize embryogenesis is key to plant development, tissue patterning, and reproductive success.
  • Previous studies lacked single-cell resolution for analyzing maize embryo development.

Purpose of the Study:

  • To describe a protocol for high-resolution analysis of maize embryogenesis.
  • To enable the study of gene regulatory networks during embryonic development.
  • To provide insights into the homology, ontogeny, and morphogenesis of embryonic organs.

Main Methods:

  • Detailed protocol for preparing maize embryo samples.
  • Application of single-cell RNA sequencing (scRNA-seq).
  • Utilization of Visium spatial transcriptomic analysis for library construction.

Main Results:

  • Enables single-cell level analysis of maize embryogenesis within a spatial context.
  • Facilitates the study of transcriptomic relationships between tissues and organs.
  • Provides a foundation for understanding gene regulatory networks during corn development.

Conclusions:

  • The described protocol effectively prepares maize embryo samples for scRNA-seq and spatial transcriptomics.
  • This combinatorial approach offers powerful tools for high-resolution maize embryogenesis research.
  • Optimized methods enhance our understanding of early plant development and gene regulation.