Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Amplified DNA sequences in cancers.

H Nakatani, E Tahara, H Sakamoto

    Biochemical and Biophysical Research Communications
    |July 31, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Coamplification of cyclin-d, hst-1 and int-2 genes is a good biological marker of high malignancy for human esophageal carcinomas.

    Oncology reports·2011
    Same author

    Expression of e-cadherin, alpha-catenins and Beta-catenins in human gastric carcinomas - correlation with histology and tumor progression.

    Oncology reports·2011
    Same author

    Increased expression and phosphorylation of sh-ptp2 (syp) in human gastric carcinomas.

    Oncology reports·2011
    Same author

    A splice variant of the c-met proto-oncogene is the predominant population expressed in human gastric mucosa and carcinoma.

    Oncology reports·2011
    Same author

    Genetic abnormalities and expression of p53 in human colon carcinomas.

    International journal of oncology·2011
    Same author

    Biologic effect of human hepatocyte growth-factor on human gastric-carcinoma cell-lines.

    International journal of oncology·2011
    Same journal

    The plasma membrane H<sup>+</sup>-ATPase OSA2 negatively regulates salt tolerance in rice seedlings.

    Biochemical and biophysical research communications·2026
    Same journal

    MiR-425-5p modulation of CREB1 affects inflammatory response and motor recovery after spinal cord injury.

    Biochemical and biophysical research communications·2026
    Same journal

    Xanthoangelol, a chalcone from Angelica keiskei, induces apoptosis in cervical cancer cell lines via the oxidative stress-mediated activation of caspase-8 and caspase-9.

    Biochemical and biophysical research communications·2026
    Same journal

    Suchilactone ameliorates podocyte injury in membranous nephropathy by modulating the JNK/p38 MAPK pathway in vitro.

    Biochemical and biophysical research communications·2026
    Same journal

    Palmitic acid-induced metabolic stress alters differentiation-associated gene expression in human ameloblast-like cells.

    Biochemical and biophysical research communications·2026
    Same journal

    A transferrin receptor-based vector enables robust Type-II membrane protein display on mammalian cells.

    Biochemical and biophysical research communications·2026
    See all related articles

    Researchers discovered significant gene amplification beyond known oncogenes in cancer cell lines using a novel in-gel DNA renaturation technique. This method revealed over 400-fold amplification in neuroblastoma, retinoblastoma, and lung carcinoma cells.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Cancer Research

    Background:

    • Gene amplification is a key mechanism in cancer development.
    • Identifying amplified genes beyond known oncogenes is crucial for understanding tumorigenesis.

    Purpose of the Study:

    • To analyze gene amplification beyond known oncogenes in specific cancer cell lines.
    • To evaluate the efficacy of an in-gel DNA renaturation method for detecting gene amplification.

    Main Methods:

    • Utilized an in-gel DNA renaturation technique involving radioactively labeled tracer DNA and unlabeled driver DNA.
    • Electrophoresis and subsequent denaturation-renaturation cycles within the gel were employed.
    • Amplified DNA fragments were visualized after two cycles.

    Related Experiment Videos

    Main Results:

    • Demonstrated over 400-fold amplification of various DNA fragments in NB1 (neuroblastoma), Y79 (retinoblastoma), and H69 (small cell lung carcinoma) cell lines.
    • Confirmed 120 to 160-fold amplification of the N-myc gene in these same cell lines.
    • The in-gel renaturation method successfully identified significant gene amplification events.

    Conclusions:

    • The study highlights the presence of substantial gene amplification beyond established oncogenes in these cancer types.
    • The in-gel DNA renaturation method is effective for discovering novel amplified DNA regions in cancer genomics.
    • Further investigation into these amplified genes may reveal new therapeutic targets.