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Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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Related Experiment Video

Updated: May 10, 2025

Author Spotlight: Establishing CENP-E Knockout HeLa Cells &#8211; A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors
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Author Spotlight: Establishing CENP-E Knockout HeLa Cells – A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors

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CRISPR Technology and Its Emerging Applications.

Xuejing Zhang1, Dongyuan Ma2, Feng Liu1,2

  • 1Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, Qingdao 266237, China.

Genomics, Proteomics & Bioinformatics
|April 23, 2025
PubMed
Summary
This summary is machine-generated.

Clustered regularly interspaced short palindromic repeats (CRISPR) technology offers efficient genome editing for biological research and clinical treatments. This review covers CRISPR-Cas systems, novel advancements, and applications in screening, diagnosis, and gene therapy.

Keywords:
CRISPRGene functionGene therapyGenome editingLineage tracing

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Clustered regularly interspaced short palindromic repeats (CRISPR) systems have revolutionized genome editing.
  • CRISPR technology enables precise manipulation of genomic elements with high efficiency and programmability.

Purpose of the Study:

  • To provide a comprehensive overview of the development and applications of CRISPR technology.
  • To describe primary and novel CRISPR-Cas systems and their diverse research and clinical uses.

Main Methods:

  • Review of CRISPR-Cas systems including CRISPR-associated protein 9 (Cas9), Cas12a, and Cas13.
  • Exploration of CRISPR applications in genetic screening, lineage tracing, genetic diagnosis, and gene therapy.

Main Results:

  • Detailed description of three primary CRISPR-Cas systems (Cas9, Cas12a, Cas13) and emerging systems.
  • Identification of CRISPR's broad applicability in various biological and medical fields.

Conclusions:

  • CRISPR technology holds transformative potential for elucidating biological mechanisms and developing clinical treatments.
  • The ongoing evolution of CRISPR promises significant advancements in studying gene functions and treating human diseases.