Bioactive Glass Microscaffolds Fabricated by Two-Photon Lithography

Porous scaffolds made of bioactive glass (BG) are of great interest for tissue engineering as they can bond to bone rapidly and promote new bone formation. Pores and channels between 100 and 500 µm provide space for cell intrusion and nutrient supply, facilitating bone ingrowth and vascularization. Furthermore, smaller pores and structural features of a few microns in size influence cell behavior, such as adhesion and osteogenic differentiation. Additive manufacturing (AM) is well suited to fabricate such geometries. However, microstructuring BG is demanding and common AM techniques are unable to achieve features below 100 µm. In this work, two-photon lithography (TPL) is used for the first time to structure BG with single-micron features. A composite containing BG nanoparticles is structured using TPL and thermally processed to receive glass scaffolds. The glass used in this study demonstrates in vitro bioactivity in simulated body fluid (SBF) and cytocompatibility toward human mesenchymal stromal cells (MSCs), making it a suitable material for tissue engineering. This process will open a toolbox for a variety of existing BG particles to be shaped with features as small as 6 µm and will broaden the understanding of the influence of scaffold design on cell behavior.