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Ec W: A Novel Narrow-Spectrum Class IIb Microcin from Escherichia coli.

Shakti K Bhattarai1,2, Vanni Bucci1,2,3, Benedikt M Mortzfeld4,5

  • 1Department of Microbiology, University of Massachusetts Chan Medical School, Worcester, MA, USA.

Probiotics and Antimicrobial Proteins
|April 24, 2025
PubMed
Summary

Researchers discovered Ec W, a novel microcin from Escherichia coli, effective against drug-resistant pathogens. This finding offers a promising alternative to traditional antibiotics for combating antimicrobial resistance.

Keywords:
Escherichia coliBacterial ecologyClass IIb microcinsLive biotherapeutics

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Area of Science:

  • Microbiology
  • Bacteriology
  • Drug Discovery

Background:

  • Antimicrobial resistance is a growing global health threat, necessitating novel therapeutic approaches.
  • Class IIb microcins are ribosomally synthesized bacteriocins that modulate bacterial communities by targeting iron acquisition.
  • The gastrointestinal tract is a competitive environment where microcins play a significant role.

Purpose of the Study:

  • To describe and characterize a novel class IIb microcin, designated Ec W, from Escherichia coli strain NCTC10444.
  • To investigate the antimicrobial activity of Ec W against various enteric pathogens.
  • To assess the potential of Ec W as a therapeutic agent against multidrug-resistant bacteria.

Main Methods:

  • Sequence analysis of Escherichia coli strain NCTC10444 to identify microcin biosynthesis clusters.
  • Heterologous expression of the novel microcin Ec W.
  • Antimicrobial inhibition assays against a panel of enteric pathogens.

Main Results:

  • Escherichia coli strain NCTC10444 harbors two homologs of the class IIb microcin biosynthesis cluster and encodes four class IIb microcins.
  • Ec W exhibits sequence similarity to class IIb microcin Gq W, expanding the known microcin class repertoire to 18.
  • Ec W demonstrated potent antimicrobial activity against drug-resistant and hypervirulent strains of E. coli and Klebsiella pneumoniae.

Conclusions:

  • Ec W is a novel class IIb microcin with significant antimicrobial activity against key enteric pathogens.
  • Ec W shows promise as a potential alternative to conventional antibiotics for treating infections caused by multidrug-resistant bacteria.
  • Exploring microcins represents a vital strategy in combating the increasing threat of multidrug-resistant bacterial infections.