Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Effects of Circadian Period Misalignment on Odontogenesis.

Stem cells and development·2026

Same author

Canadian Professional Association Resources on Diet and Tooth Decay: Website Content Analysis.

JMIR formative research·2026

Same author

Preparation and Characterization of Alginate-Based Bioinks for Three-Dimensional Bioprinting of Cell-Laden Constructs.

Current protocols·2025

Same author

Emergency zero-fluoroscopy catheter ablation for refractory ventricular tachycardia in third-trimester pregnancy: a case report.

Frontiers in cardiovascular medicine·2025

Same author

Corrigendum to 'Collecting and deactivating TGF-β1 hydrogel for anti-scarring therapy in post-glaucoma filtration surgery' [Mater. Today Bio, 14 (2022) 100260].

Materials today. Bio·2025

Same author

Corrigendum to "A dual-transduction-integrated biosensing system to examine the 3D cell-culture for bone regeneration" [Biosens. Bioelectron. 141 (2019) 111481].

Biosensors & bioelectronics·2025

Search research articles

Related Experiment Video

Updated: May 10, 2025

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Published on: October 10, 2013

Competitive enzyme linked aptamer based assay for salivary melatonin detection.

Meenakshi Pundir^1,2,3, Liubov Lobanova², Petros Papagerakis^4,5,6

¹Centre interprofessionnel pour la recherche clinique et appliquée (CIRCA), Faculty of Dentistry, Université Laval, Dental Medicine Pavilion, 2420, rue de la Terrasse, Quebec City, G1V0A6, Canada.

Scientific Reports

|April 24, 2025

Summary

A new aptamer-based assay accurately measures melatonin in saliva, aiding the diagnosis of circadian rhythm disorders. This method offers high sensitivity for detecting low melatonin levels, crucial for personalized sleep disorder treatments.

More Related Videos

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Measuring Biophysical and Psychological Stress Levels Following Visitation to Three Locations with Differing Levels of Nature

Measuring Biophysical and Psychological Stress Levels Following Visitation to Three Locations with Differing Levels of Nature

Published on: June 19, 2019

Related Experiment Videos

Last Updated: May 10, 2025

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Published on: October 10, 2013

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Measuring Biophysical and Psychological Stress Levels Following Visitation to Three Locations with Differing Levels of Nature

Measuring Biophysical and Psychological Stress Levels Following Visitation to Three Locations with Differing Levels of Nature

Published on: June 19, 2019

Area of Science:

Biochemistry
Biotechnology
Chronobiology

Background:

Melatonin regulates sleep-wake cycles and circadian rhythm.
Accurate measurement of dim light melatonin onset (DLMO) is crucial for diagnosing sleep-wake disorders.
Traditional melatonin detection methods lack sensitivity for precise DLMO assessment.

Purpose of the Study:

To develop a novel, highly sensitive assay for melatonin detection in saliva.
To utilize aptamers for specific and sensitive melatonin quantification.
To improve the accuracy of DLMO determination for circadian rhythm disorder diagnosis.

Main Methods:

Development of a competitive enzyme-linked aptamer-based assay.
Utilizing chemically synthesized DNA/RNA aptamers for melatonin binding.
Assessing assay performance including linear dynamic range and detection limit.

Main Results:

The aptamer assay demonstrated high specificity and sensitivity for melatonin.
Achieved a linear dynamic range from 8.62 × 10⁻⁶ M to 3.9 × 10⁻¹¹ M.
Established a low detection limit of 2.5 × 10⁻¹² M (~0.57 pg/mL) with good recovery in diluted saliva.

Conclusions:

The aptamer-based assay shows potential for accurate low-level melatonin detection in saliva.
This method can significantly aid in precise DLMO determination, especially in vulnerable populations.
Improved DLMO measurement facilitates optimized diagnosis and personalized treatment of circadian rhythm disruptions.