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Terpenoids from Chloranthus holostegius with Anti-inflammatory Activities
GuoCai Wang1,2, Zhaochun Zhan1, Ting Lu1
1State Key Laboratory of Bioactive Molecules and Druggability Assessment, and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM & New Drugs Research College of Pharmacy, Jinan University, Guangzhou, P. R. China.
Abstract:
Five new terpenoids, including one isocamphane-type monoterpenoid (1), one labdane-type diterpenoid (2), and three cadinane-type sesquiterpenoids (3‒5), were isolated from the whole plant of Chloranthus holostegius. A combination of spectral analysis (HR-ESI-MS, UV, IR, and 1D/2D NMR), quantum chemical calculations (NMR/ECD), and X-ray crystallography were employed in the structural characterization. In anti-inflammatory assays, all isolates were evaluated by examination of their inhibitory effects on nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells, and compounds 1 and 5 exhibited moderate inhibitory activity against NO release, with IC50 values of 21.16 ± 1.37 and 18.03 ± 1.24 µM, respectively.
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