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Related Experiment Video

Updated: May 10, 2025

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
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Exploring Hesperidin Hydrogel for Pulp Capping Advancements: An In Vitro Study.

Swetha Geervani V1, Kiran Kumar Neelakantappa1, Manimozhi M1

  • 1Department of Conservative Dentistry and Endodontics, Government Dental College and Research Institute, Bengaluru, IND.

Cureus
|April 25, 2025
PubMed
Summary
This summary is machine-generated.

Hesperidin hydrogel shows superior sustained release of vascular endothelial growth factor (VEGF) compared to Biodentine, indicating greater potential for regenerative endodontics and direct pulp capping. This natural flavonoid offers promising long-term pulp vitality.

Keywords:
biodentinehesperidinhydrogelspulp capping and pulpectomyregenerative endodonticsvascular endothelial growth factor (vegf)

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Area of Science:

  • Biomaterials Science
  • Endodontics
  • Regenerative Medicine

Background:

  • Regenerative endodontics aims to preserve pulp vitality using bioactive materials for dentinogenesis.
  • Traditional pulp-capping agents (PCAs) like Biodentine have limitations in long-term bioactivity.
  • Hesperidin, a natural flavonoid, exhibits anti-inflammatory and angiogenic properties, making it a potential alternative.

Purpose of the Study:

  • To compare the growth factor release profiles of hesperidin hydrogel and Biodentine.
  • To assess their potential in vital pulp therapy and direct pulp capping.

Main Methods:

  • Sixty human third molars were used to create dentin discs, divided into hesperidin hydrogel and Biodentine groups.
  • Vascular endothelial growth factor (VEGF) release was quantified using ELISA over 7, 14, and 28 days.
  • In vitro permeation and scanning electron microscopy (SEM) analyses were conducted.

Main Results:

  • Hesperidin hydrogel exhibited significantly higher and sustained VEGF release than Biodentine across all time points (p < 0.0001).
  • Biodentine showed an initial burst release followed by a decline, while hesperidin hydrogel maintained stable bioactivity.
  • Hesperidin hydrogel demonstrated prolonged regenerative potential.

Conclusions:

  • Hesperidin hydrogel is a promising next-generation biomaterial for direct pulp capping due to its sustained VEGF release.
  • It promotes angiogenesis and supports long-term pulp vitality, potentially revolutionizing regenerative endodontics.
  • Further in vivo studies are warranted to confirm clinical efficacy.