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Updated: Jun 26, 2026
Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
Published on: March 7, 2018
Dendritic Gold Nanoparticles Loaded on 3D Graphene-like Surface and Layer-by-Layer Assembly for Enhanced Glucose
Zifeng Zhu1, Yiming Zhao1, Yongming Ruan2
1College of Chemistry and Materials Science, Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, Zhejiang Normal University, Jinhua 321004, China.
Background/Objectives:
In this study, AuDNs/EPLE composite electrodes with hierarchical dendritic nanogold structures were fabricated using the in situ electrodeposition of gold nanoparticles through the i-t method.
Methods:
A conductive polymer composite membrane, PEDOT, was synthesized via the electropolymerization of EDOT and the negatively charged PSS-. The negatively charged SO3- groups on the surface of the PEDOT membrane were electrostatically adsorbed with the glucose oxidase (GOD) enzyme and a positively charged chitosan co-solution (GOD/chit+). Using a layer-by-layer self-assembly approach, GOD was incorporated into the multilayers of the composite electrode to create the composite GOD/chit+/PEDOT/AuDNs/EPLE.
Results:
Electrochemical analysis revealed a GOD surface coverage of 8.5 × 10-10 mol cm-2 and an electron transfer rate of 1.394 ± 0.02 s-1. The composite electrode exhibited a linear response to glucose in the concentration range of 6.923 × 10-2 mM to 1.54 mM, with an apparent Michaelis constant of 0.352 ± 0.02 mM. Furthermore, the GOD/chit+/PEDOT/AuDNs/EPLE also showed good accuracy of glucose determination in human serum samples.
Conclusions:
These findings highlight the potential of the GOD/chit+/PEDOT/AuDNs/EPLE composite electrode in the development of efficient enzymatic biofuel cells for glucose sensing and energy harvesting applications.
