Electric Field-Induced Effects in Eukaryotic Cells: Current Progress and Limitations

Electric fields (EFs) offer a powerful tool for manipulating cells and modulating their behavior, holding significant promise for regenerative medicine and cell biology. We provide a comprehensive overview of the effects of different types of EF on eukaryotic cells with the special focus on physical mechanisms and signaling pathways involved. Direct current EF induces electrophoresis and electroosmosis, influencing cell migration, proliferation, and differentiation. Alternating current EF, through dielectric polarization and dielectrophoresis, enables cell manipulation, trapping, and sorting. Pulsed EF, particularly high-intensity, short-duration pulses, induces reversible and irreversible electroporation, facilitating drug and gene delivery. The review covers some technological aspects of EF generation, emphasizing the importance of experimental setups, and integration with microfluidic platforms for high-throughput analysis and precise manipulations. Furthermore, the synergistic potential of combining EFs with optical tweezers is highlighted, enabling fine-tuned control of cell positioning, intercellular interactions, and measurement of biophysical properties. Finally, the review addresses limitations of EF application, such as field heterogeneity and potential side effects, and outlines the directions for future studies, including developing the minimally invasive delivery methods.Impact StatementThe application of electric fields (EFs) for cell manipulation and modulation of cellular functions and behavior is a promising task in regenerative medicine and cell biology. This article provides systemized and structured information about EF parameters, induced effects in cells in vitro, and involved signaling pathways and introduces novice biomedical engineers to research in this field.