Proliferation of human embryonic corneal stromal cells in a serum-free medium

Substantial multiplication of stromal cells from human embryonic corneas has been obtained in a basal medium MCDB 104 supplemented with 25 ng EGF/ml, 10 micrograms insulin/ml, 20 micrograms transferrin/ml, 25 ng MSA/ml, 500 micrograms ovalbumin/ml, 50 micrograms LDL/ml, 50 micrograms HDL/ml and 10(-6) M hydrocortisone. Even though the growth rate appears to be similar to that in 10% serum, the cells cease proliferating at a lower density.