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TULP3 Regulates Proliferation and Differentiation of 3T3-L1 Preadipocytes Through the Hedgehog Signaling Pathway

Xinlin Jin1, Yu Zhang1, Yunzhou Wang2

  • 1Shandong Provincial Key Laboratory for Livestock Germplasm Innovation & Utilization, College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China.

Biology
|April 26, 2025
PubMed

Insights

Tubulin polymerization-depolymerizing protein 3 (TULP3) promotes adipocyte differentiation by inhibiting the Hedgehog signaling pathway. This finding offers insights into treating adipose tissue dysfunction and related diseases.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The TULP family, including TULP3, is known from obese mice studies and tumor cell research.
  • The specific role of TULP3 in adipocytes and its mechanism of action in adipose tissue remain largely unexplored.

Purpose of the Study:

  • To investigate the role and mechanism of TULP3 in the differentiation of adipocytes.
  • To determine if TULP3 influences adipogenesis via the Hedgehog signaling pathway.

Main Methods:

  • Utilized 3T3-L1 cells to study TULP3 expression during adipocyte differentiation.
  • Manipulated TULP3 expression (overexpression and inhibition) to assess its impact on cell proliferation and differentiation.
  • Investigated the interaction between TULP3 and the Hedgehog signaling pathway in adipogenesis.

Main Results:

  • TULP3 expression increased during 3T3-L1 cell differentiation.
  • Overexpression of TULP3 enhanced adipocyte proliferation and differentiation, while inhibition had the opposite effect.
  • TULP3 was identified as a negative regulator of the Hedgehog signaling pathway, promoting adipogenic differentiation by inhibiting pathway activity.

Conclusions:

  • TULP3 plays a crucial role in promoting adipogenic differentiation of precursor adipocytes.
  • Inhibition of the Hedgehog signaling pathway by TULP3 is a key mechanism in adipogenesis.
  • These findings provide valuable information for understanding adipocytogenesis and developing treatments for adipose tissue dysfunction and related diseases.
Keywords:
Hedgehog signaling pathwayTULP3adiposeprecursor adipocytes

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