Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

17.0K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
17.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanism of UV-C-Induced Oxygen Vacancies Altering the Colour of Dental Zirconia.

Materials (Basel, Switzerland)·2026
Same author

Droplet-Engineered Scalable High-Throughput Perovskite Micropatterning for Next-Generation Optoelectronics.

ACS nano·2026
Same author

Injectable carbon dioxide controlled releasing and photothermal carboxymethyl chitosan-alginate-black tea carbon hydrogel dressing for diabetic wound healing.

Regenerative biomaterials·2026
Same author

Label-Free Detection of Nuclear Envelope Nucleoporation using 2D Morphological Embeddings and Machine Learning.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Fucoxanthin-Loaded Solid Lipid Nanoparticles Exert Potent Therapeutic Efficacy in Combating High-Fat Diet Induced Obesity in Mice.

International journal of molecular sciences·2025
Same author

Nanoparticle-Reinforced Hydrogel with a Well-Defined Pore Structure for Sustainable Drug Release and Effective Wound Healing.

ACS applied bio materials·2025

Related Experiment Video

Updated: May 10, 2025

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
10:44

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

6.4K

Pico-Scale Digital PCR on a Super-Hydrophilic Microarray Chip for Multi-Target Detection.

Qingyue Xian1,2, Jie Zhang2, Yu Ching Wong3

  • 1Academy of Interdisciplinary Studies, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon 999077, Hong Kong.

Micromachines
|April 26, 2025
PubMed
Summary

This study introduces a new super-hydrophilic microarray chip for digital polymerase chain reaction (dPCR). The innovative design improves efficiency and enables precise quantification of targets like hepatitis B virus (HBV) DNA.

Keywords:
POCTdPCRhydrophilicmicroarray

More Related Videos

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
09:32

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

Published on: June 9, 2011

9.9K
Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

7.2K

Related Experiment Videos

Last Updated: May 10, 2025

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
10:44

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

6.4K
ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
09:32

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

Published on: June 9, 2011

9.9K
Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

7.2K

Area of Science:

  • Biotechnology
  • Microfluidics
  • Molecular Diagnostics

Background:

  • Current digital polymerase chain reaction (dPCR) technologies face challenges with device size, sample loading, and reaction unit consistency.
  • Droplet merging and limited partition sizes hinder the efficiency and accuracy of existing dPCR platforms.

Purpose of the Study:

  • To develop a novel super-hydrophilic microarray chip for digital polymerase chain reaction (dPCR).
  • To overcome limitations of existing dPCR devices by enabling streamlined sample loading and uniform reaction unit formation.
  • To demonstrate the chip's capability for absolute quantitative detection of various nucleic acid targets.

Main Methods:

  • Designed a super-hydrophilic microarray chip utilizing micro-electro-mechanical systems (MEMS) technology.
  • Employed hydrodynamic principles for uniform 120-pL reaction unit formation within a closed channel.
  • Integrated rapid thermal cycling and real-time imaging for efficient dPCR analysis.

Main Results:

  • Achieved absolute quantitative detection of hepatitis B virus (HBV) plasmids across different concentrations.
  • Successfully performed multiplexed detection of cancer mutation gene fragments and reference genes.
  • Demonstrated a uniform array of independent reaction units, indicating high assay efficiency.

Conclusions:

  • The developed super-hydrophilic microarray chip offers a streamlined and efficient platform for digital polymerase chain reaction (dPCR).
  • The technology shows significant potential for sensitive and accurate molecular diagnostics.
  • This platform is well-suited for point-of-care testing (POCT) applications, particularly in cancer diagnostics.