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Published on: December 10, 2014
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Anaerobic Digestion of High-Solid Chicken Manure (CM) at Different Temperature: Intestinal Microbiome Efficiency,
Xujing Chen1, Qigui Niu1,2,3, Jingyi Li1
1China-America CRC for Environment & Health, School of Environmental Science and Engineering, Shandong University, Qingdao 266237, China.
Microorganisms
|April 26, 2025
Summary
High solids and temperature impact anaerobic digestion of chicken manure. High free ammonia and volatile fatty acids reduce methane but increase VFA accumulation, especially without inoculation.
Area of Science:
- * Environmental Microbiology
- * Biochemical Engineering
- * Waste Management
Background:
- * Anaerobic digestion (AD) of high-solid chicken manure (CM) offers resource recovery potential.
- * The influence of substrate overload and temperature on AD performance and microbial processes remains poorly understood.
- * Limited data exists on the metabolic pathways during CM digestion with and without inoculation.
Purpose of the Study:
- * To investigate the effects of temperature, total solids (TS), and inoculation on AD of high-solid CM.
- * To analyze the metabolic variations and microbial community shifts under different conditions.
- * To establish a scientific basis for optimizing CM waste resource utilization.
Main Methods:
- * Experimental design involving four temperatures (4 °C, 35 °C, 55 °C, 75 °C), varying TS, and inoculation treatments.
- * Characterization of AD performance, including methane (CH4) production and volatile fatty acids (VFAs) accumulation.
- * Utilization of EEM-FRI (Excitation-Emission Matrix-Fluorescence Regional Integration) for analyzing coenzyme F420 and microbial community analysis.
Main Results:
- * High temperatures increased coenzyme F420, while TS influenced microbial production.
- * Combined high TS and temperature led to high free ammonia (FA) and free volatile fatty acids (FVFA), inhibiting CH4 production and promoting VFA accumulation (up to 12 g/L at 55 °C).
- * Intestinal microbiota alone achieved 11 g/L VFA; cross-feeding symbiosis enhanced CH4 production (68 mL/gVS at 35 °C); high temperatures favored heat-resistant bacteria and reduced microbial diversity.
Conclusions:
- * Temperature and TS significantly affect the acidification efficiency of high-solid CM digestion.
- * Inoculation strategy and microbial symbiosis play crucial roles in enhancing methane production.
- * Findings provide critical insights for optimizing anaerobic digestion processes for chicken manure resource utilization.
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