Comparative toxicity of eleven bisphenol analogs in the nematode Caenorhabditis elegans

Bisphenol analogs are widely used as industrial substitutes for Bisphenol A (BPA) and are included in water bottles, food containers, and receipts commonly encountered daily. However, there are currently no specific regulations on these substitute substances, and reports on their harmful effects are also lacking. In this study, we examined the toxicity of eleven bisphenol analogs, including BPAP, BPB, BPC, BPC2, BPE, BPG, BPM, BPP, BPPH, BPZ, and TBBPA at 1 mM concentration using the C. elegans model. Our findings revealed that several bisphenol analogs, most notably BPB, BPC, BPE, and BPG, significantly increased lethality in embryonic and L1 larval stages. Additionally, developmental delays were observed with BPAP, BPB, BPC, and BPG, with a reduced fraction of animals reaching adulthood. Regarding reproductive toxicity, we found that BPAP, BPB, BPC, BPC2, and BPG reduced egg production. Furthermore, exposure to the analogs significantly shortened the lifespan of C. elegans, particularly with BPAP, BPB, BPC, and BPG, raising concerns about their potential impact on aging. This study suggests their potential harmful effects on development, reproduction, and longevity.