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Updated: May 13, 2026

Genomic Transformation of the Picoeukaryote Ostreococcus tauri
Published on: July 13, 2012
Genetic manipulation of the betaproteobacterial genera Thauera and Aromatoleum
Dominik Hege1, Yvonne Gemmecker1, Lina Clermont1
1Department of Biology, Laboratory for Microbial Biochemistry, Philipps University Marburg, Marburg, Germany.
Abstract:
Most recombinant proteins are expressed in model host organisms like Escherichia coli. Meanwhile, a significant number of enzymes require complex activation or special cofactors not available from standard hosts. The betaproteobacteria Thauera and Aromatoleum allow access to some of these enzymes, following procedures described in this chapter. The methods described enable transformation and conjugation of vectors into these species as alternate gene expression systems which allow fundamental studies of complex recombinant proteins as well as their biotechnological application.
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