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Assessing the application of landmark-free morphometrics to macroevolutionary analyses.

James M Mulqueeney1,2, Thomas H G Ezard3, Anjali Goswami4

  • 1School for Ocean & Earth Science, National Oceanography Centre Southampton, University of Southampton Waterfront Campus, Southampton, UK. j.m.mulqueeney@soton.ac.uk.

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Automated, landmark-free shape analysis shows promise for large-scale evolutionary studies. While efficient, these methods require further refinement for broad adoption across diverse taxa.

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Geometric morphometricsLandmark-free morphometricsMacroevolutionMammaliaMorphometricsShape

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Area of Science:

  • Evolutionary biology
  • Morphometrics
  • Computational anatomy

Background:

  • 3D geometric morphometrics revolutionized phenotypic evolution studies.
  • Manual landmarking is time-consuming, operator-dependent, and limits cross-taxa comparisons.
  • Automated, landmark-free methods offer potential but are underexplored for macroevolutionary analyses.

Purpose of the Study:

  • To evaluate automated, landmark-free approaches for macroevolutionary analyses.
  • To compare Deterministic Atlas Analysis (DAA) with high-density geometric morphometrics.
  • To assess the utility of these methods across diverse mammalian taxa.

Main Methods:

  • Applied Large Deformation Diffeomorphic Metric Mapping (LDDMM) via DAA.
  • Utilized a dataset of 322 mammals from 180 families.
  • Standardized mixed-modality scan data (CT and surface scans) using Poisson surface reconstruction.

Main Results:

  • Standardization improved DAA-manual landmarking correspondence, though differences persisted for specific taxa (Primates, Cetacea).
  • Both DAA and manual methods yielded comparable, yet varying, estimates for phylogenetic signal, morphological disparity, and evolutionary rates.
  • DAA demonstrated potential for large-scale, cross-taxa studies due to enhanced efficiency.

Conclusions:

  • Landmark-free methods like DAA offer efficiency gains for macroevolutionary morphometrics.
  • Challenges remain, particularly regarding accuracy across morphologically disparate taxa.
  • Further research and methodological improvements are needed for widespread adoption of landmark-free analyses.