Bioengineered nanomaterials for dynamic diagnostics in vivo

In vivo diagnostics obtains real-time physiological information directly from the site of interest in a patient's body, providing more accurate disease diagnosis compared with ex vivo diagnostics. Particularly, in vivo dynamic diagnostics allows the continuous monitoring of physiological signals over a period of time, offering deeper insights into disease pathogenesis and progression. However, achieving in situ dynamic diagnostics in deep tissues presents challenges related to energy and signal penetration as well as dynamic monitoring. Bioengineered nanomaterials serve as an ideal platform for in vivo dynamic diagnostics, leveraging energy conversion and biofunctionalization to enable continuous acquisition of physiological information across temporal and spatial scales. In this review, with reference to the studies from the last five years, we summarize the fundamental components that are essential for dynamic diagnosis in vivo. Firstly, an input energy source with high tissue penetration is needed, such as near-infrared (NIR) light, X-rays, magnetic field and ultrasound. Secondly, a nanomaterial class that is responsive to such an energy source to provide a readable output signal is chosen. Thirdly, bioengineered nanoprobes are designed to exhibit spatial, temporal or spatiotemporal changes in the output signal. Finally, different methods are used to analyse the output signal of nanoprobes, such as detecting changes in optical, radiation, magnetic and ultrasound signals. This review also discusses the obstacles and potential solutions for advancing these bioengineered nanomaterials toward clinical translational applications.