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Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies
Published on: July 13, 2013
The Effect of Xenon on the Voltage‒Current Characteristics of Tethered Lipid Bilayers
Hadeel Alobeedallah1, Bruce Cornell2, Hans Coster3
1School of Chemical and Biomolecular Engineering, The University of Sydney, Sydney, 2006, Australia. haal5646@uni.sydney.edu.au.
Abstract:
In this study, we describe the effect of the noble gas, xenon on the electrical properties of tethered lipid bilayer membranes, (tBLMs), including the effect of xenon on the activation energy for electrical conduction through the tBLM. Such studies benefit from the stability of a tethered membrane given the wide range of temperatures that are scanned and the time required for these measurements. The results indicate that xenon increases the activation energy for electrical conduction through bilayers and decreases the average pore size that dominates the electrical conductance of the lipid bilayers at low voltages. Xenon possesses a high affinity for lipid membranes and is a potent general anaesthetic. Its anaesthetic potency is possibly associated with its effects on proteins embedded in the lipid membranes.
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