Hypoxia-inducible factor 1α expression does not differ between canine urothelial carcinoma and normal urinary bladder
Nicole H Gibbs1, Mario F Sola2, Deepika Dhawan3
1Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA.
American Journal of Veterinary Research
|April 28, 2025
Summary
Hypoxia-inducible factor-1α (HIF-1α) is not differently expressed in canine urothelial carcinoma (UC). Further research is needed to explore other hypoxia-related proteins in canine UC tumor growth.
Area of Science:
- Veterinary Oncology
- Molecular Biology
- Cancer Research
Background:
- Hypoxia-inducible factor-1α (HIF-1α) plays a critical role in cellular response to hypoxia.
- Understanding HIF-1α expression in canine urothelial carcinoma (UC) is important for potential therapeutic targets.
Purpose of the Study:
- To compare the RNA and protein expression of HIF-1α in canine UC versus normal urinary bladder tissue.
- To investigate the role of HIF-1α in canine urothelial carcinoma.
Main Methods:
- Tissue samples from dogs with UC and healthy controls were analyzed.
- Immunohistochemistry was used to assess HIF-1α protein expression (IRS).
- RNA sequencing evaluated HIF-1α gene expression.
Main Results:
- No significant difference in intracytoplasmic HIF-1α protein expression was found between UC and normal tissues (median IRS, 1 for both).
- RNA sequencing revealed no significant difference in HIF-1α expression between the groups (1.3-fold change).
Conclusions:
- This study found no differential expression of HIF-1α at the RNA or protein level in canine UC.
- Further investigation into other hypoxia-associated proteins is warranted for canine UC tumor growth and treatment resistance.
