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A cell line, ROSE 199, derived from normal rat ovarian surface epithelium
Experimental Cell Biology
|January 1, 1985
Summary
A rat ovarian surface epithelium cell line (ROSE 199) forms papillary structures mimicking human ovarian tumors. This suggests ovarian surface epithelium may be the origin of common ovarian epithelial tumors.
Area of Science:
- Cell Biology
- Oncology
- Gynecology
Background:
- Ovarian epithelial tumors are common and their origin is hypothesized to be the ovarian surface epithelium.
- The ROSE 199 cell line was derived from rat ovarian surface epithelium (ROSE).
Purpose of the Study:
- To investigate the characteristics of the ROSE 199 cell line and its resemblance to ovarian tumors.
- To explore the potential of ROSE 199 cells to shed viable cells and produce collagen.
Main Methods:
- Culturing the ROSE 199 cell line under crowded conditions.
- Histological examination of papillary structures formed by ROSE 199 cells.
- Observation of cell shedding and collagen production in culture.
Main Results:
- ROSE 199 cells formed papillary structures histologically similar to human serous papillary cystadenomas of borderline malignancy.
- Crowded cultures of ROSE 199 cells exhibited two cell layers separated by collagen and shed viable cells.
- ROSE 199 cells retained epithelial morphology while expressing stromal activity, including collagen production.
Conclusions:
- The ROSE 199 cell line's resemblance to ovarian tumors supports the hypothesis that ovarian epithelial tumors originate from the ovarian surface epithelium.
- The dual epithelial and stromal behavior of ROSE 199 cells may contribute to the neoplastic transformation of ovarian surface epithelium.