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Author Spotlight: Assessing the Cardiovascular Profile of Patients with Metabolic Syndrome
Published on: September 27, 2024
Comparison of plasma metabolic profiling between children with hypertrophic obstructive cardiomyopathy and healthy
Shuo Dong1, Chuhao Du1, Hao Cui1,2
1Department of Pediatric Cardiac Surgery, National Center for Cardiovascular Disease and Fuwai Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College.
Insights
Serum metabolomics reveals significant differences in children with hypertrophic obstructive cardiomyopathy (HOCM). These findings aid in understanding HOCM pathogenesis and early diagnosis in pediatric patients.
Area of Science:
- Cardiovascular Research
- Metabolomics
- Pediatric Cardiology
Background:
- Congenital heart disease diagnosis can be aided by serum metabolomics.
- Metabolomic differences between pediatric hypertrophic obstructive cardiomyopathy (HOCM) and healthy children are not well understood.
- This study investigates the metabolomic profiles of children with HOCM.
Purpose of the Study:
- To identify distinct serum metabolite profiles in children diagnosed with HOCM.
- To explore potential biomarkers for early HOCM detection in pediatric populations.
- To elucidate the metabolic pathways affected in HOCM.
Main Methods:
- Targeted metabolomic analysis was performed on fasting blood samples.
- Samples were collected from 24 children with HOCM, 11 with non-HOCM left ventricular outflow tract obstruction, and 41 healthy controls.
- Ultra-performance liquid chromatography coupled with mass spectrometry (UPLC-MS) was utilized.
Main Results:
- Significant alterations in 79 out of 224 measured plasma metabolites were observed between HOCM and control groups.
- No significant metabolic differences were found between HOCM and non-HOCM groups.
- Enriched metabolic pathways in HOCM patients included purine and thiamine metabolism.
Conclusions:
- Significant plasma metabolite changes distinguish children with HOCM from healthy controls.
- These identified metabolic alterations contribute to understanding HOCM's underlying mechanisms.
- The findings support the potential of metabolomics for early HOCM diagnosis in children.
Background:
The diagnostic value of serum metabolomics in congenital heart disease has been proven. The difference of serum metabolomics between children with hypertrophic obstructive cardiomyopathy (HOCM) and normal children is unknown.
Methods:
Fasting blood samples of 24 symptomatic children with HOCM, 11 children with left ventricular outflow tract obstruction because of other cardiac anomalies (non-HOCM group) and 41 normal controls were obtained. The targeted metabolomic approach was performed using a Vanquish ultra-performance liquid chromatography system coupled to a Q-Exactive HF mass spectrometer.
Results:
The plasma level of 79 out of 224 metabolites were significantly changed (|log2FC| > 1 and P adj. < 0.05) between the HOCM and the normal group. There was no significant difference between the HOCM and non-HOCM groups. A total of 79 significantly changed metabolites between the HOCM and the normal group were significantly enriched in two pathways, including purine metabolism (P < 0.001) and thiamine metabolism (P = 0.034).
Conclusion:
Our results identified the significant changes of plasma metabolite between children with HOCM and healthy children, which is helpful for understanding the pathogenesis of HOCM and early diagnosis.
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