Antioxidant analysis and quality standardization of Scutellaria baicalensis tablets using multidimensional analytical...

Area of Science:

• Analytical Chemistry
• Natural Product Chemistry
• Food Science

Background:

• Scutellaria baicalensis Tablets (SBTs) show potential as natural antioxidants for functional foods.
• Accurate quality control is crucial for utilizing SBTs effectively.

Purpose of the Study:

• To develop and validate an integrated analytical workflow for comprehensive evaluation of SBT quality and antioxidant capacity.
• To establish a robust foundation for precise quality control of SBTs.

Main Methods:

• Utilized electronic-transition-theory-driven multi-wavelength concatenation for enhanced flavonoid detection.
• Employed a binary evaluation system (BES) with S_m and P_m parameters for multidimensional quality appraisal.
• Integrated dual-channel high-performance liquid chromatography-online antioxidant activity coupling (DC-HPLC-OAA) and electrochemical profile analysis.

Main Results:

• Multi-wavelength concatenation overcame single-wavelength detection limitations, capturing subtle inter-flavonoid variations.
• The BES demonstrated robust correlation with key component levels (e.g., baicalin), validated by chemometric analysis.
• DC-HPLC-OAA systematically screened antioxidant activities of major flavonoids, quantified by electrochemical integration.

Conclusions:

• The integrated workflow provides precise quality control for Scutellaria baicalensis Tablets.
• SBTs show significant promise as natural antioxidant ingredients for functional food applications.