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Related Concept Videos

CRISPR01:59

CRISPR

48.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
48.5K
CRISPR and crRNAs02:53

CRISPR and crRNAs

16.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.3K

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Related Experiment Video

Updated: May 9, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

960

Advances in CRISPR/Cas13a-based biosensors for non-coding RNA detection.

Tao Zhu1, Weiwei Jiang1, Yingyu Wu1

  • 1Department of Preventive Medicine, Zhejiang Provincial Key Laboratory of Pathological and Physiological Technology, School of Medicine, Ningbo University, Ningbo, 315000, China.

Talanta
|April 29, 2025
PubMed
Summary

CRISPR/Cas13a biosensors offer a sensitive and rapid method for detecting non-coding RNAs, overcoming limitations of traditional techniques. These advanced tools promise improved clinical diagnostics and personalized medicine applications.

Keywords:
CRISPR/Cas13a-based biosensorElectrochemistryFluorescenceSurface-enhanced Raman spectroscopyncRNA

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DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
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Last Updated: May 9, 2025

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DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Diagnostics

Background:

  • Non-coding RNAs are critical in disease pathogenesis and serve as potential biomarkers.
  • Conventional RNA detection methods (PCR, NGS, ELISA, smFISH, nanopore, scRNA-seq) have limitations like complexity and long processing times.
  • Efficient detection of regulatory non-coding RNAs is crucial for diagnostics.

Purpose of the Study:

  • To review CRISPR/Cas13a-based biosensors for non-coding RNA detection.
  • To explore various signal transduction systems integrated with CRISPR/Cas13a.
  • To highlight advancements in portable detection devices and future clinical applications.

Main Methods:

  • Overview of CRISPR/Cas13a RNA detection mechanisms.
  • Analysis of CRISPR/Cas13a biosensor development.
  • Integration of biosensors with fluorescence, electrochemical, colorimetric, and SERS detection methods.

Main Results:

  • CRISPR/Cas13a biosensors demonstrate high potential for sensitive and specific RNA detection.
  • Integration with diverse signal transduction systems enhances diagnostic capabilities.
  • Progress in portable platforms like lateral flow assays and smartphone-based systems facilitates point-of-care applications.

Conclusions:

  • CRISPR/Cas13a biosensors represent a significant advancement over conventional methods for non-coding RNA analysis.
  • These biosensors are poised to revolutionize clinical diagnostics and personalized medicine.
  • Further development is needed to address current challenges for widespread clinical adoption.