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DNAzyme-based colorimetric biosensor for rapid detection of Shigella flexneri
Manikandan Murugan1, Elangovan Saranya1, Mohandass Ramya2
1Molecular Genetics Laboratory, Department of Genetic Engineering, SRM Institute of Science and Technology, College of Engineering and Technology, Kattankulathur, Chengalpattu, Chennai, Tamil Nadu, 603 203, India.
Scientific Reports
|April 29, 2025
Summary
A new colorimetric assay rapidly detects Shigella flexneri, a dangerous bacterium causing shigellosis. This DNAzyme-based method using aptamers and carbon nanotubes offers a user-friendly tool for food and water safety.
Area of Science:
- Microbiology
- Nanotechnology
- Biotechnology
Background:
- Shigella flexneri causes shigellosis, a severe diarrheal disease.
- Current detection methods are slow and inconvenient.
- Rapid diagnostics are crucial for food and water safety.
Purpose of the Study:
- Develop a rapid, accurate diagnostic assay for S. flexneri.
- Utilize aptamers and DNAzyme technology for detection.
Main Methods:
- Modified S. flexneri-specific HGD-aptamer and single-walled carbon nanotubes (SWCNTs).
- Developed a colorimetric detection assay exploiting DNAzyme activity.
- Validated assay sensitivity, specificity, and compared with PCR.
Main Results:
- Achieved a detection limit of 51 CFU/mL for S. flexneri.
- Demonstrated selective detection with no signal interference from other bacteria.
- Assay performance comparable to PCR methods.
Conclusions:
- The developed assay is a rapid, cost-effective, and user-friendly tool for S. flexneri detection.
- Potential to enhance food safety, environmental monitoring, and public health surveillance.
- Innovative approach for pathogen identification.
