Palm Multidiagnostic of Mycoplasma pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, and Streptococcus

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Xingchi Kan1, Yue Wu1, Xiyue Zhang2

  • 1Zhejiang Lab Hangzhou 311121China.

Transboundary and Emerging Diseases
|April 30, 2025
PubMed

Insights

A new diagnostic method, Palm CRISPR/Cas12a Diagnostic (PaCD), rapidly detects four common respiratory pathogens, including Mycoplasma pneumoniae. This point-of-care test offers accurate results in 30 minutes, aiding clinical decisions.

Area of Science:

  • Molecular Biology
  • Infectious Diseases
  • Biotechnology

Background:

  • Rising incidence of Mycoplasma pneumoniae (Mp) infections necessitates rapid diagnostic tools.
  • Accurate detection of respiratory pathogens is crucial for effective clinical management.
  • Existing diagnostic methods may lack speed or point-of-care accessibility.

Purpose of the Study:

  • To develop a rapid, accurate, and point-of-care diagnostic method for Mycoplasma pneumoniae and other key respiratory pathogens.
  • To evaluate the performance of the developed diagnostic method using clinical samples.
  • To assess the potential of 3D printing for creating portable diagnostic devices.

Main Methods:

  • Development of a one-tube Palm CRISPR/Cas12a Diagnostic (PaCD) assay.
  • Utilizing 3D printing to create a compact, portable detection device with temperature control and blue light irradiation.
  • Testing PaCD with sputum and throat swab samples, comparing results with next-generation sequencing and CT scans.

Main Results:

  • PaCD achieved a detection limit of 50 copies/test and provided results within 30 minutes.
  • The method successfully detected Mycoplasma pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, and Streptococcus pneumoniae.
  • PaCD showed 100% concordance with next-generation sequencing and high concordance with CT scan results.

Conclusions:

  • The PaCD method offers a rapid and accurate point-of-care solution for diagnosing infections caused by four prevalent respiratory pathogens.
  • The integration of 3D printing enhances the portability and feasibility of the diagnostic device.
  • PaCD holds promise for versatile applications in detecting various respiratory pathogens in diverse clinical settings.

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