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Design and Biosynthesis of Ornithine 8-Containing Semaglutide Variants with a Click Chemistry-Modifiable Position 26.

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Summary

Researchers developed a novel method to create semaglutide variants for improved type 2 diabetes and obesity treatments. This approach enhances drug properties and facilitates high-throughput screening for better patient outcomes.

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OspRRiPPsamber stop codon incorporationornithinesemaglutide

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Area of Science:

  • Medicinal Chemistry
  • Biotechnology
  • Peptide Synthesis

Background:

  • Semaglutide, a GLP-1 receptor agonist, is vital for type 2 diabetes and obesity management.
  • Current limitations include poor oral bioavailability, side effects, and high costs, hindering patient adherence.
  • Optimizing semaglutide structure is key to overcoming these challenges.

Purpose of the Study:

  • To develop a rapid method for generating diverse semaglutide mutants for high-throughput screening.
  • To address limitations of current semaglutide treatments by optimizing peptide structure.
  • To create a broad library of semaglutide analogues with potentially improved properties.

Main Methods:

  • Utilized a novel
  • Fits-In-All
  • approach combining RiPP technology and amber stop codon incorporation.
  • Incorporated noncanonical amino acid ornithine at position 8 using OspR to prevent DPP-4 cleavage.
  • Employed an orthogonal tRNA/aminoacyl-tRNA synthetase pair for click chemistry-based functionalization at position 26.

Main Results:

  • Successfully generated a diverse library of semaglutide variants.
  • Demonstrated a method for site-specific incorporation of noncanonical amino acids and functional groups.
  • Enabled the creation of semaglutide analogues for screening of optimized properties.

Conclusions:

  • The developed method offers a novel pathway for semaglutide analogue synthesis.
  • This approach facilitates the design of optimized peptide-based GLP-1 receptor agonists.
  • Opens new avenues for improving semaglutide's therapeutic potential in diabetes and obesity.