Related Experiment Video
Updated: May 9, 2025
Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit
Published on: December 16, 2015
Development of a novel procedure for detecting bloody milk based on an objective quantification using opal-glass
Takashi Sugawara1, Shige Koseki2, Kento Koyama2
1Graduate School of Agricultural Science, Hokkaido University, Kita-9, Nishi-9, Kita-ku, Sapporo 060-8589, Japan; Manufacturing Support Division, Tokachi Industrial Development Center, Tokachi-foundation, 23-9, Kita2-chome, Nishi22-jo, Obihiro-city, Hokkaido 080-2462, Japan.
Abstract:
This study aimed to enhance the reliability of blood-milk detection by quantifying hemoglobin levels in raw milk through visible light spectroscopy combining the sodium lauryl sulfate-hemoglobin and opal-glass methods. Bulk milk samples were collected from the northern (Area A), southern (Area B), and central (Area C) parts of the Tokachi region of Hokkaido from August 2023 to March 2024. Bovine blood was added to milk to make blood-milk samples. A calibration curve was generated using blood milk samples (n = 201) collected from Area C. Blood milk samples collected from Areas A (n = 112) and B (n = 126), were prepared as test sets. A hemoglobin concentration threshold was 0.1 g/L, where identifying bloody milk visually becomes challenging. The calibration curve demonstrated high binary-classification accuracy for bloody milk in Areas A and B, with sensitivities of 93.3 % and 97.2 % and specificities of 94.0 % and 95.6 %.
More Related Videos
04:16Individualized Reconstitution of Human Milk Microbiota: A Feasible Approach in Real-World Settings
Published on: February 7, 2025
12:50Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water
Published on: June 9, 2014