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Related Experiment Video

Updated: May 9, 2025

Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization
09:14

Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization

Published on: October 20, 2019

9.6K

Lipoaspiration and Processing to Create Microfragmented Adipose Tissue.

Cally McGee1, Michael R Baria1, Corey Gatewood2

  • 1Department of Physical Medicine and Rehabilitation, The Ohio State University, Columbus, Ohio, USA.

Video Journal of Sports Medicine
|May 1, 2025
PubMed
Summary
This summary is machine-generated.

This video demonstrates adipose harvest and preparation for microfragmented adipose tissue (MFAT) to treat musculoskeletal conditions. Learn this simple technique for autologous cell therapy in orthopedics.

Keywords:
lipoaspiratemicrofragmented adipose tissueorthobiologicsosteoarthritistendinopathy

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Related Experiment Videos

Last Updated: May 9, 2025

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Area of Science:

  • Orthobiologics
  • Regenerative Medicine
  • Musculoskeletal (MSK) Interventions

Background:

  • Orthobiologics are gaining traction for treating MSK conditions.
  • Adipose tissue is a potential source of autologous cells for orthobiologic applications.
  • Current orthopedic training often lacks comprehensive instruction in adipose harvest and processing techniques.

Purpose of the Study:

  • To provide a detailed review of the technique for adipose harvest and preparation.
  • To demonstrate the creation of microfragmented adipose tissue (MFAT) from lipoaspirate.
  • To educate orthopedic professionals on a valuable adjunctive procedure for MSK pathology.

Main Methods:

  • The procedure involves local anesthesia and tumescent solution injection.
  • Adipose tissue is harvested using a 17-gauge trochar and a double-syringe system.
  • The harvested lipoaspirate undergoes decanting, rinsing with sterile saline, and resizing through a filter to produce MFAT.

Main Results:

  • Microfragmented adipose tissue (MFAT) can be efficiently prepared using lipoaspiration.
  • The technique yields a unique population of autologous cells.
  • The described method is suitable for both clinic and operating room settings.

Conclusions:

  • Lipoaspirate is a straightforward technique for generating MFAT.
  • MFAT offers a source of autologous cells for potential MSK condition treatment.
  • This method enhances the utility of adipose tissue in orthobiologic interventions.