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Related Concept Videos

Equipments Used to Measure Body Temperature

Equipments Used to Measure Body Temperature

Body temperature can be assessed using various devices and measured in Celsius or Fahrenheit.
Glass-bulb Thermometer:
Glass-bulb thermometers are hollow glass tubes with a bulb tip containing liquid such as ethanol or mercury. Historically, glass bulb mercury thermometers were the standard device to measure body temperature. Today, mercury thermometers are prohibited in many countries due to the hazardous effects of mercury and the risk of exposure if the glass bulb breaks. In general,...

Body Temperature

Body Temperature

The body's temperature, measured in degrees, is determined by the balance between heat production and dissipation to the surrounding environment. For instance, if exercising vigorously, the body will produce more heat, causing sweat and dissipating that heat. Despite extreme environmental conditions and physical exertion, the human temperature-control system maintains a constant core body temperature (the temperature of deep tissues, which are the tissues located beneath the skin and other...

Thermosensation

Thermosensation

Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...

Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

Assessing Body Temperature - Oral

Assessing Body Temperature - Oral

Here are the steps to accurately measure oral temperature using an electronic thermometer:
Step 1:
Start by practicing proper hand hygiene to prevent the spread of microorganisms.
Step 2:
Take the thermometer out of the charging unit, switch it on, and wait for the ready sign.
Step 3:
Gently slide the probe cover until a click is heard. This simple action prevents cross-contamination and ensures the correct placement of the probe cover.
Step 4:
Instruct the patient to open their mouth and place...

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Related Experiment Video

Updated: May 9, 2025

Measuring Skeletal Muscle Thermogenesis in Mice and Rats

Measuring Skeletal Muscle Thermogenesis in Mice and Rats

Published on: July 27, 2022

Genetically-encoded temperature indicators for thermal biology.

Tetsuichi Wazawa¹, Ryohei Ozaki-Noma^1,2, Lu Kai¹

¹SANKEN (The Institute of Scientific and Industrial Research), Osaka University, Ibaraki, Osaka 567-0047, Japan.

Biophysics and Physicobiology

|May 1, 2025

Summary

This summary is machine-generated.

Genetically-encoded temperature indicators (GETIs) are protein-based fluorescent nanothermometers for observing temperature in living biological samples. This review covers GETI mechanisms, development, and characterization for thermal biology research.

Keywords:

GETI GFP-like protein fluorescence microscopy nanothermometer

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Last Updated: May 9, 2025

Measuring Skeletal Muscle Thermogenesis in Mice and Rats

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Area of Science:

Biophysics
Molecular Biology
Thermal Biology

Background:

Temperature is a critical physical parameter influencing molecular processes and biological functions.
Macroscopic sensors are inadequate for measuring temperature at microscopic scales (e.g., cells, organelles).
Fluorescent nanothermometers offer a solution for non-invasive temperature measurements in living biological systems.

Purpose of the Study:

To review protein-based fluorescent nanothermometers, also known as genetically-encoded temperature indicators (GETIs).
To provide an overview of GETI sensing mechanisms, measurement methods, and development strategies.
To propose characterization standards and discuss future perspectives in thermal biology.

Main Methods:

Review of published literature on genetically-encoded temperature indicators (GETIs).
Analysis of temperature sensing mechanisms and fluorescence microscopy techniques.
Examination of protein engineering approaches for GETI development.

Main Results:

Comprehensive overview of various protein-based fluorescent nanothermometers (GETIs).
Detailed explanation of temperature sensing principles and measurement methodologies.
Identification of unique protein engineering techniques for GETI construction.

Conclusions:

GETIs are valuable tools for non-invasive temperature monitoring in living biological samples.
Standardized characterization is crucial for reliable GETI development and application.
Further research in GETIs will advance the field of thermal biology.