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Protocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and
Stina Oredsson1, Atena Malakpour-Permlid2, Johan Zhu3
1Department of Biology, Lund University, 22362 Lund, Sweden.
STAR Protocols
|May 1, 2025
Abstract:
The Oredsson universal replacement (OUR) medium is the formulation of a universal xeno-free medium designed for the cultivation of human normal and cancer cells in 2D and 3D cultures. Here, we present a protocol for the use of OUR medium for routine culturing, cell banking, and medium modification for suspension culture. We describe steps for thawing, sub-culturing, and freezing cells. We then detail procedures for using these techniques for VERO, A549, THP-1, and Jurkat cells.

