A portable sweat biosensor for multiple chronic kidney diseases biomarkers detection

Area of Science:

• Electrochemistry
• Biosensor Technology
• Biomarker Detection

Background:

• Chronic kidney disease (CKD) management requires continuous monitoring of toxin biomarkers.
• Current sweat analysis methods for CKD lack sensitivity, specificity, and durability.
• Non-invasive detection methods are crucial for patient convenience and proactive disease management.

Purpose of the Study:

• To develop a novel molecularly imprinted portable biosensor for simultaneous detection of urea, creatinine, and uric acid in human sweat.
• To overcome limitations of conventional biosensors, including sensitivity, specificity, and long-term stability.
• To provide a convenient and accurate tool for point-of-care diagnosis and personalized management of CKD.

Main Methods:

• Fabrication of a flexible biosensor on PET film using screen-printing technology.
• Development of ternary composite electrodes (NiCoMOF/MWCNTs/NCDs) for enhanced electrochemical properties.
• Application of molecular imprinting technology to create specific recognition cavities for target biomarkers.

Main Results:

• The biosensor demonstrated high sensitivity and low detection limits for urea, creatinine, and uric acid.
• Achieved excellent linearity (R² > 0.995) across physiological concentration ranges.
• Maintained signal retention above 95.8% after 60 days, indicating long-term durability.
• Successfully performed synchronous dynamic monitoring of three biomarkers in sweat samples.

Conclusions:

• The developed molecularly imprinted portable biosensor offers a promising solution for non-invasive CKD biomarker monitoring.
• This technology enables continuous, convenient, and accurate point-of-care diagnosis.
• The biosensor has high potential for personalized management strategies in chronic kidney diseases.