Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Allosteric Regulation01:08

Allosteric Regulation

57.2K
Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...
57.2K
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

4.3K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.3K
Epigenetic Regulation01:37

Epigenetic Regulation

3.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.0K
Histone Modification02:32

Histone Modification

12.9K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
12.9K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

8.1K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
8.1K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

6.7K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
6.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual-Pathway Catalytic Proton Exchange in Water Distillation Enables Record Detritiation of Tritiated Water.

Environmental science & technology·2026
Same author

Fitness-for-Service Assessment of Dent Defects on Steel Energy Pipelines: Evaluation Criteria, Integrity Prediction, and Future Challenges.

Materials (Basel, Switzerland)·2026
Same author

Thermal runaway-free Na-ion batteries.

National science review·2026
Same author

Precision Electronic Tuning of Gold Nanoclusters: A Generalizable Strategy for Enhanced Hydrogen Evolution Electrocatalysis via Platinum Doping.

Inorganic chemistry·2026
Same author

The effects of exercise interventions on bone mineral density in middle-aged and older men: a systematic review and meta-analysis.

Frontiers in physiology·2026
Same author

Cumulative exposure and patterns of the cholesterol-HDL-C-glucose index and risk of new-onset cardiometabolic multimorbidity: 9-year longitudinal evidence from CHARLS.

Frontiers in nutrition·2026
Same journal

UPF3A and UPF3B shape the transcriptome cooperatively yet oppose cell function.

Journal of molecular biology·2026
Same journal

Antibody-secreting cells integrate efficient NMD with non‑canonical UPR signaling to maintain proteostasis and support massive immunoglobulin synthesis.

Journal of molecular biology·2026
Same journal

Small molecule stabilization of diverse amyloidogenic immunoglobulin light chains revealed by hydrogen-deuterium exchange mass spectrometry.

Journal of molecular biology·2026
Same journal

UPF1 at Work: Structural and Mechanistic Insights Into a Master Regulator of Nonsense-Mediated mRNA Decay.

Journal of molecular biology·2026
Same journal

Structural basis for the pro-amyloidogenic action and ligand binding of a novel W72R variant of human apolipoprotein A-I.

Journal of molecular biology·2026
Same journal

Cryo-EM Structure of the C. elegans Septin Tetramer Reveals a Revised Architecture and Conserved Positional Orthology.

Journal of molecular biology·2026
See all related articles

Related Experiment Video

Updated: May 16, 2025

Author Spotlight: Epigenetic Modifications and Metabolic Rewiring as Targets for Cancer Therapy
07:20

Author Spotlight: Epigenetic Modifications and Metabolic Rewiring as Targets for Cancer Therapy

Published on: October 18, 2024

423

Deciphering Allosteric Modulation of Cancer-Associated Histone Missense Mutations.

Shuxiang Li1, Jie Shu2, James C Rober3

  • 1Department of Pathology and Molecular Medicine, Queen's University, ON, Canada.

Journal of Molecular Biology
|May 1, 2025
PubMed
Summary
This summary is machine-generated.

Cancer-associated histone mutations can disrupt normal cell function through allosteric modulation. This study identified specific mutations affecting chromatin dynamics and genome stability, suggesting a role in cancer development.

Keywords:
allosteric regulationallosterycancerchromatin dynamicshistone mutationsmutationnucleosome

More Related Videos

Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue
08:12

Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue

Published on: May 5, 2022

3.8K
Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

6.5K

Related Experiment Videos

Last Updated: May 16, 2025

Author Spotlight: Epigenetic Modifications and Metabolic Rewiring as Targets for Cancer Therapy
07:20

Author Spotlight: Epigenetic Modifications and Metabolic Rewiring as Targets for Cancer Therapy

Published on: October 18, 2024

423
Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue
08:12

Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue

Published on: May 5, 2022

3.8K
Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

6.5K

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Cancer Research

Background:

  • Histone mutations are linked to cancer, but their impact on chromatin dynamics is unclear.
  • Understanding these mechanisms is crucial for cancer epigenetics research.

Purpose of the Study:

  • To investigate the allosteric modulation effects of 40 cancer-associated histone missense mutations.
  • To assess the functional impact of these mutations on chromatin and genome stability.

Main Methods:

  • Computational approaches were used to predict allosteric effects.
  • Experimental validation was performed for specific histone mutations (H2BS64Y, H2BS64F).

Main Results:

  • Allosteric effects of histone mutations are position-specific, with N-terminal tail mutations causing significant perturbations.
  • Seven mutations were predicted to alter nucleosome interactions.
  • Experimental mutations disrupted histone function, H2BK120 ubiquitination, and genome stability.

Conclusions:

  • Allostery is a potential mechanism driving the oncogenic effects of some histone mutations.
  • Further research into allosteric pathways in cancer epigenetics is warranted.