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Enhancing the Brightness of Red-emitting Fluorophores in Aqueous Solution by Molecular Encapsulation
Liza Briant1, Alexandre Fürstenberg2
1Department of Physical Chemistry and Department of Inorganic and Analytical Chemistry, University of Geneva, CH-1211 Genève 4, Switzerland. liza.briant@unige.ch.
Abstract:
Fluorescence spectroscopy and microscopy in biomolecular environments are usually performed in aqueous solution and preferably using red-emitting dyes. However, water quenches their fluorescence. We explore in this contribution how host-guest interactions between red-emitting fluorophores and macrocycles such as cyclodextrins and cucurbiturils can prevent quenching by shielding the dyes from water, thereby enhancing their brightness. We successfully apply this strategy in super-resolution imaging.
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