Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-institution experience of 500 consecutive pancreatoduodenectomies: case-matched outcomes of the da Vinci Xi robotic versus open procedures.

Surgical endoscopy·2026
Same author

Automated serial electron diffraction: implementation in <i>LibraEDT</i> and its applications.

Journal of applied crystallography·2026
Same author

Adenosine Receptor Functionality and Desensitization Machinery in a Neuronal Cell Model of Angelman Syndrome.

Journal of developmental biology·2026
Same author

Fluid shear stress enhances pancreatic cancer motility through YAP activation.

Clinical epigenetics·2026
Same author

The emerging role of AXL in pancreatic cancer: Biomarker potential and therapeutic targeting to counteract drug resistance.

Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy·2026
Same author

The multidimensional signature of oxysterols: Comparative RPLC-HRMS-based profiling of healthy and pancreatic tumour cell lines.

Talanta·2026

Related Experiment Video

Updated: May 9, 2025

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media
08:32

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

Published on: February 12, 2019

13.6K

Novel PPT+SEC Workflow for High-Sensitivity Extracellular Vesicle Proteomics from Cell Media.

Asia Botto1,2, Chiara De Cesari1,3, Noa Ndimurwanko1,4

  • 1Fondazione Pisana per la Scienza ONLUS, 56017 San Giuliano Terme (PI), Italy.

Journal of Proteome Research
|May 2, 2025
PubMed
Summary

This study introduces a novel extracellular vesicle (EV) isolation method for proteomics, reducing sample loss and improving protein identification. The streamlined workflow enhances sensitivity for cancer biomarker discovery.

Keywords:
EVsexosomesextracellular vesiclespancreatic cancerproteomicssize-exclusion chromatography

More Related Videos

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
09:16

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Published on: January 7, 2019

9.7K
Purification of High Yield Extracellular Vesicle Preparations Away from Virus
00:07

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Published on: September 12, 2019

11.3K

Related Experiment Videos

Last Updated: May 9, 2025

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media
08:32

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

Published on: February 12, 2019

13.6K
Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
09:16

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Published on: January 7, 2019

9.7K
Purification of High Yield Extracellular Vesicle Preparations Away from Virus
00:07

Purification of High Yield Extracellular Vesicle Preparations Away from Virus

Published on: September 12, 2019

11.3K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Proteomics

Background:

  • Extracellular vesicles (EVs) isolation using size exclusion chromatography (SEC) typically requires a concentration step.
  • This concentration step can lead to significant loss of valuable EV material.
  • Current methods limit the efficiency of EV proteomics analysis.

Purpose of the Study:

  • To develop a novel EV isolation workflow compatible with direct proteomics analysis.
  • To minimize EV loss during sample preparation.
  • To enhance the sensitivity and scope of EV proteomics.

Main Methods:

  • Development of a low-volume EV isolation technique using size exclusion chromatography (SEC).
  • Characterization of isolated small EVs via transmission electron microscopy, Western blot, and nanoparticle tracking analysis.
  • Proteomics analysis of isolated EVs and benchmarking against an automated UHPLC-SEC platform.

Main Results:

  • The novel workflow isolates EVs in 80 microL, eliminating the need for concentration.
  • Identified more proteins and EV markers compared to UHPLC-SEC, including 96% of top exosomal proteins from ExoCarta.
  • Demonstrated higher sensitivity for pancreatic cancer EV markers in pancreatic cancer cell lines.

Conclusions:

  • The developed EV isolation method is efficient and preserves EV integrity for proteomics.
  • This approach significantly enhances the identification of EV proteins and biomarkers.
  • The workflow shows promise for sensitive detection of cancer-specific EV markers.