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Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

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Published on: February 9, 2024

Specific ATP Detection Using Molecule-Responsive DNA Nanopores.

Hiromu Akai¹, Taichi Hirano², Takuya Mabuchi³

¹Department of Mechanical Engineering, Nagaoka University of Technology, 1603-1 Kamitomioka, Nagaoka, Niigata, 940-2188, Japan.

Small (Weinheim an Der Bergstrasse, Germany)

|May 3, 2025

Summary

Researchers developed a novel DNA nanopore sensor that can detect adenosine triphosphate (ATP) using a molecule-responsive aptamer. This breakthrough enables precise single-molecule measurements for potential applications in drug discovery and disease diagnostics.

Keywords:

DNA nanopores aptamers lipid bilayers molecular dynamics simulation nanopore sensing sensors

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Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

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Area of Science:

Biotechnology
Nanotechnology
Molecular Biology

Background:

Nanopore sensing offers label-free, high-resolution single-molecule analysis.
Conventional nanopores have limitations in functionalization and molecular selectivity.
Synthetic DNA nanopores present superior designability and functionalization potential.

Purpose of the Study:

To develop a functionalized DNA nanopore for stochastic sensing.
To create a molecule-responsive nanopore for detecting specific analytes.
To demonstrate adenosine triphosphate (ATP) detection using a novel DNA nanopore sensor.

Main Methods:

Constructed a synthetic DNA nanopore using structural DNA nanotechnology.
Integrated an adenosine triphosphate (ATP)-binding split DNA aptamer into the nanopore.
Utilized the aptamer's binding and dissociation with ATP to control pore opening and closing.
Performed stochastic nanopore sensing experiments to detect ATP.

Main Results:

Successfully developed a molecule-responsive DNA nanopore.
Demonstrated repeated opening and closing of the nanopore entrance triggered by ATP binding and dissociation.
Achieved successful ATP measurements via stochastic nanopore sensing with the functionalized DNA nanopore.

Conclusions:

Developed a novel ATP-responsive DNA nanopore sensor.
Achieved stochastic nanopore sensing using functionalized DNA nanopores for the first time.
Tailored DNA nanopore sensors hold promise as analytical tools for drug discovery and disease diagnosis.