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Updated: May 9, 2025
A Hydrogel Construct and Fibrin-based Glue Approach to Deliver Therapeutics in a Murine Myocardial Infarction Model.
Published on: June 14, 2015
A bioactive hydrogel patch accelerates revascularization in ischemic lesions for tissue repair.
Zhuo Liu1, Kang Wu1,2, Hong Zeng1
1Department of Physiology and Pathophysiology, School of Basic Medical Sciences, State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, 138 Xueyuan Road, Shanghai 200032, P.R. China.
A novel magnesium-releasing adhesive patch (MgAP) promotes vascular regeneration and tissue repair in ischemic conditions. This ion therapy approach offers a targeted delivery of magnesium ions (Mg2+) for improved cardiac and limb function.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Cardiovascular Research
Background:
- Magnesium ions (Mg2+) are vital for cellular functions and promote angiogenesis, showing potential for cardiovascular ischemic diseases.
- Conventional Mg2+ administration faces challenges like systemic side effects, low bioavailability, and lack of targeted delivery.
- A magnesium-releasing adhesive tissue patch (MgAP) was developed for sustained, localized Mg2+ release.
Purpose of the Study:
- To develop and evaluate a novel Mg2+-releasing adhesive tissue patch (MgAP) for targeted ion therapy.
- To assess the efficacy of MgAP in promoting vascular regeneration and tissue repair in models of myocardial infarction and lower limb ischemia.
- To elucidate the underlying mechanisms of MgAP's proangiogenic and therapeutic effects.
Main Methods:
- Ionic crosslinking was used to fabricate the MgAP, with its structure and properties confirmed by FTIR and rheological analysis.
- Sustained Mg2+ release was quantified over 7 days using ICP-MS.
- Efficacy was evaluated in rat models of myocardial infarction and lower limb ischemia, using echocardiography, strain analysis, histological staining, in vitro/in vivo angiogenesis assays, transcriptomics, and Western blotting.
Main Results:
- MgAP application significantly improved cardiac function and reduced adverse remodeling in myocardial infarction rats compared to intravenous MgCl2.
- Key improvements included increased ejection fraction (20.3%) and radial strain (27.4%), with reduced fibrosis and apoptosis.
- Proangiogenic effects were confirmed in vitro and in vivo, with MgAP also demonstrating efficacy in a lower limb ischemia model.
Conclusions:
- The MgAP demonstrated a significant vascular regeneration effect through localized Mg2+ delivery via ion therapy.
- This novel hydrogel strategy effectively aids cardiac and lower limb repair in ischemic tissues.
- The MgAP presents a promising therapeutic approach for various ischemic conditions.
