The effect of 50 Hz magnetic fields on cellular sensitivity of mouse spermatogenic cell lines to hydrogen peroxide

With the widespread application of electromagnetic technology, electromagnetic fields (EMFs) emitted from various electric and electronic devices have significantly altered the electromagnetic environment. This has raised concerns about the potential health impacts of EMFs. Previous studies have indicated that EMFs may influence male infertility, with oxidative stress proposed as a key factor; however, the underlying mechanisms remain unclear. In this study, we aimed to determine whether EMFs enhance the impact of oxidative stress on male infertility. We investigated the effects of 50 Hz magnetic fields (MFs) on the sensitivity of mouse spermatogenic cell lines (GC-1 spg and GC-2 spd) to low-dose hydrogen peroxide (H₂O₂, 5 and 10 μM). Our findings revealed that pre-exposure to 2.0 mT 50 Hz MFs for 24 h increased the sensitivity of GC-2 spd cells to low-dose H₂O₂ in terms of γH2AX foci formation, a marker for DNA damage repair. However, no significant changes were observed in DNA fragmentation, cell viability, or cell cycle progression in either GC-1 spg or GC-2 spd cells. In conclusion, our results suggest that 50 Hz MFs do not significantly enhance the sensitivity of mouse spermatogenic cell lines to low-dose H₂O₂.